DuoWave® Rocking Single-use Bioreactor

The unique mixing method of DuoWave® wave bioreactor provides a gentle growth environment for cells. The newly designed process control software has various functions and a simple and intuitive interface, which is easy to get familiar. The working volume of DuoWave® wave bioreactor ranges from 500 ml to 25 L, which is suitable for the seed train expansion, inoculum preparation, culture process development of various types of cells and fast preparation of laboratory samples.

Main functions

DuoWave® wave bioreactor adopts a new modular design, which consists of four modules: base, tray, I/O and server. It is easy to install, operate and saves space.

The base module is designed with functions of temperature measurement, rocking and mixing, weighing and exhaust gas heating. After installing the tray, it can be started by setting the LCD touch screen that comes with the base.

The tray includes two specifications of 20L and 50L, and adopts a dual-zone heating plate design, which can realize independent temperature control of two cell culture bags.

The I/O module integrates optical pH, DO electrodes, gas mixing and high-precision peristaltic pump functions, and users can easily realize process parameter feedback control.

The server module includes DuoWave® control software and computer, through which users can perform process control, audit trail, data recording, four-level authority management, batch report generation and other operations. DuoWave® wave bioreactor system complies with GMP and 21 CFR Part 11 requirements.

Applications

Depending on the mode of operation, DuoWave® wave bioreactors can be applied to

Seed Expansion

Fed-Batch

Perfusion

Microcarrier

DuoWave® Rocking Single-use Bioreactor

Depending on different applications, DuoWave® wave bioreactors can be applied to

Antibody and recombinant proteins

Vaccine

Gene therapy

Cell therapy

Other biologics or macro molecular

Mass transfer and mixing performance

DuoWave® wave bioreactor system not only has excellent mass transfer and mixing performance, but also brings extremely low shear force and suitable growth environment for cell culture due to its unique non-sparging aeration and non-stirring design.

Component configuration and part number

Module	Main module model	Configuration	Parameters
Base	Base	Temperature control module	Double-plate independent temperature control, continuously adjustable from RT to 42°C, PID closed-loop control, water-cooled bottom plate can be customized.
		Rocking mixing module	Rocking angle is continuously adjustable from 0-12°, and the speed is continuously adjustable from 0-23 rpm
		Weighing Module	0 – 50 kg, can cascade with peristaltic pump
		2 exhaust gas heaters	80 ± 10 °C automatic constant temperature
Tray	Tray20	2 separate temperature measuring and heating plates	Compatible with DuoWave® 3 L, 10 L, 20 L cell culture bags
Tray	Tray50	2 separate temperature measuring and heating plates	Compatible with DuoWave® 22 L, 50 L cell culture bags
I/O Module	I/O module	Two Watson-Marlow 114D variable speed peristaltic pumps; Optical DO and pH sensors Gas mixing device	Peristaltic pump flow rate range 0-5mLPM（13#）, 0-22mLPM（14#）, 0-80mLPM（16#）, 0-200mLPM（25#）. The measure range of DO and pH are 0-200% and 5-9, respectively. The gas flowrate range of gas 0-1 LPM, with CO₂ range of 0-20% and O₂ range of 0-90%.
Server Module	Microsoft Surface	Microsoft Surface Pro7 Built-in DuoWave® control software	i5, 8G+128G, standard + Pro original keyboard
	Laptop	Dell laptop Built-in DuoWave® control software	i5, 8G or above RAM, 512G or above SSD
	computer	Dell PC Built-in DuoWave® control software	i5 , 8G memory, 512G or above solid state drive; Windows 10 or Windows 11 Professional Edition

	Dimension	Weight
Base	470560264 mm	27.7 kg
20L Tray	79253562 mm	10.7 kg
50L Tray	90468874 mm	16.9 kg
20L Tray（w/ cooling）	79253565 mm	15.4 kg
50L Tray（w/ cooling）	904*688-77 mm	21.9 kg
20L top cover	832549274 mm	4.9 kg
50L top cover	904688307 mm	6.3 kg
I/O module	310418185 mm	10.8 kg
pH optical fiber	1.5 m
DO optical fiber	1.5 m

Base	Performance parameter
Rocking speed	0-40 rpm
Rocking angle	0-12°
Weighing range	0-50 kg
Weighing accuracy	10 g
Temperature electrode	Pt100
Temperature measurement range	0-80 °C
Temperature measurement accuracy	±0.1 °C
Temperature control range	10°C below room temperature (w/ cooling tray), room temperature to 45°C
Temperature control accuracy	±0.2 °C

I/O Module	Performance parameter
Gas flowrate range	Air 0.3 L/min CO₂ 0.3 L/min*20% O₂ 0.3 L/min
Gas flowrate accuracy	0.5% of full-scale
Fast filling gas flow rate	3 LPM
CO₂control range	0-20%
CO₂measurement accuracy	±0.2%
CO₂control accuracy	±0.5%
O₂control range	N₂mode 0-80% & Air mode 21%-80%
O₂measurement accuracy	2%
O₂control accuracy	0.5%
pH measurement range	pH 4.5-pH 8.5
pH control range	pH 6.0-pH 8.0
pH measurement accuracy	±0.05 pH, within ±0.25 pH of the calibration value ±0.1 pH, ±0.25 to ±0.5 pH from calibration value
pH control accuracy	±0.05 pH
DO measurement range	0-250%
DO control range	0-100%
DO measurement accuracy	±5%

Peristaltic Pump	Performance parameter
Number of peristaltic pumps	2* Watson-Malow 114DV
Flowrate range	0-200 mL/min
Pump accuracy	10% of total volume
Compatible tubing sizes	13# (1/32 in-5/32 in) 14# (1/16 in-3/16 in) 16# (1/8 in-1/4 in) 25# (3/16 in-5/16 in)

DuoWave® Cell Culture Bag

DuoWave® Rocking Single-use Bioreactor

Features of DuoFilm-003 film material

DuoFilm-003 is a 5-layer coextruded film that withstands the harshest physical requirements while exhibiting excellent cell culture compatibility.

The outer layer is low-density polyethylene (LDPE), which has excellent chemical stability and elongation.

The middle layer is ethylene-vinyl alcohol copolymer EVOH, which exhibits excellent barrier effect on gases, odors, solvents, etc., while maintaining flexibility and strength properties

Liquid contact layer is ultra low density polyethylene (ULDPE) for excellent biocompatibility and low extractable levels while providing a clean, inert surface with no anti-blocking additive ingredients.

We provide DuoWave® cell culture bags with various sizes and configurations for different bioprocessing applications.

Configuration	Description
Tube material	Optional silicone tube or weldable tube
pH/DO	Optional configurations with or without optical electrodes
Volume	Optional 3L, 10L, 15L, 20L, 22L, 50L
Sampling port	Luer sampling port
Film material	DuoFilm^®- 003