Duoning DuoBioX® Elite is a benchtop cell culture bioreactor system that supports dual culture modes. Users can quickly establish and develop cell culture processes ranging from 3 to 15 liters with this system, achieving process establishment, development, and optimization with a highly cost-effective investment. The DuoBioX® Elite system is ideal for the early development of cell culture processes in scientific research institutions or the biopharmaceutical industry.
Design Concept
The Elite system fully complies with the technical performance requirements of bioreactors in the biopharmaceutical industry. The process software employs a user-friendly design language to facilitate easier operation. The system operates stably and reliably, occupies minimal space, and offers users options for both touch and mobile operation platforms.
Material of Construction
The stainless-steel parts of the tank body that comes into contact with the liquid is constructed from SUS316L stainless steel and is electropolished to create an inert surface that is easy to clean. The glass tank body is made of high-quality tempered borosilicate glass, while the sealing materials consist of EPDM, silicone, and other substances that comply with FDA requirements. The non-liquid contact parts are made from high-quality SUS304 stainless steel.
System Hardware
Various tank specifications are available, including 3L, 5L, 7L, 15L and more.
The software is powerful and capable of comprehensive control based on feedback, time, conditions, and script recipes, effectively meeting the user's stringent process requirements.
The operating system, developed on the WinCC industrial-grade architecture, complies with GMP and 21 CFR Part 11 regulations, making it suitable for use in industries such as pharmaceuticals and medical devices.
In addition to the fundamental parameters of dissolved oxygen (DO), pH, temperature, liquid level, and stirring control units, the system can accommodate up to three ventilation gas pathways: surface ventilation, macro sparger bottom ventilation, and micro sparger bottom ventilation. This flexibility allows users to implement diverse and adaptable ventilation strategies, effectively supporting high-density perfusion culture processes. Furthermore, the optimized exhaust gas cooling device significantly reduces the risk of exhaust gas filter blockage and is compatible with both fermentation and high-density cell culture applications.
The controller is designed with additional input interfaces to connect sensors, such as those for cell density, CO2 levels, and weighing. It can also accommodate additional sensors in the control feedback system.
Model | Elite 500mL | Elite 3L | Elite 7L | Elite 15L |
Function | Cell culture type, 1 control 1 or 1 control 2 (optional) | |||
Tank body | ||||
Working volume | 400 mL | 2 L | 5 L | 10 L |
Total volume | 500 mL | 3.1 L | 6.8 L | 15 L |
Min. working volume | 100 mL | 0.6 L | 1.5 L | 3.2 L |
H-to-D ratio | 2:1 | 1.9:1 | 2.2:1 | 2.0:1 |
Np value | 1.5 | 1.5 | 1.5 | 1.5 |
Impeller | 3 inclined blades 45° | 3 inclined blades 45° | 3 inclined blades 45° | 3 inclined blades 45° |
Impeller diameter | 35 mm | 45 mm | 60 mm | 75 mm |
Vent pipe diameter | 4 mm | 6 mm | 8 mm | 8 mm |
Macro sparger pore size | 0.5 mm | 1 mm | 1 mm | 1 mm |
Number of macro spargers | 3 | 5 | 7 | 7 |
Micro sparger pore size | None | Optional | Optional | Optional |
Controller | ||||
pH sensor | Gel / 120 | Gel / 225 | Gel / 325 | Gel / 325 |
DO sensor | 243666-112/120 (Optical) H2 | 243666-132/225 (Optical) H2 | 243666-142/325 (Optical) H2 | 243666-142/325 (Optical) H2 |
CO2 sensor | Optional | Optional | Optional | Optional |
Overlay vent | Rotor flow meter | Rotor flow meter | Rotor flow meter | Rotor flow meter |
MFC-Air | 0~0.1 L | 0~0.5 L | 0~1 L | 0~2 L |
MFC-O2 | 0~0.1 L | 0~0.5 L | 0~1 L | 0~2 L |
MFC-CO2 | 0~0.1 L | 0~0.5 L | 0~0.5 L | 0~1 L |
MFC-N2 | Optional | Optional | Optional | Optional |
MFC-O2 micro sparger | 0~0.1 Optional | 0~0.1 Optional | 0~0.5 Optional | 0~1 Optional |
Built-in peristaltic pump | 4x built-in, viable speed | 4x built-in, viable speed | 4x built-in, viable speed | 4x built-in, viable speed |
Temperature control | Electric heating base 100w Cooling water solenoid valve | Electric heating blanket 400w Cooling water solenoid valve | Electric heating blanket 400w Cooling water solenoid valve | Electric heating blanket 400w Cooling water solenoid valve |
Exhaust gas condensation | None | Semiconductor or water cooling | Semiconductor or water cooling | Semiconductor or water cooling |
Weighting | Optional | Optional | Optional | Optional |
Control software (needs to be installed on the user's computer) | ||||
21 CFR Part 11 | Yes | Yes | Yes | Yes |
Data records | Yes | Yes | Yes | Yes |
User authorization management | 4 levels | 4 levels | 4 levels | 4 levels |
Utility requirements | ||||
Air source | Air, O2, CO2 | Air, O2, CO2 | Air, O2, CO2 | Air, O2, CO2 |
Air source pressure | 0.2-0.3 MPa | 0.2-0.3 MPa | 0.2-0.3 MPa | 0.2-0.3 MPa |
Control cabinet power supply | 220 v 50 Hz | 220 v 50 Hz | 220 v 50 Hz | 220 v 50 Hz |
Power socket | 10A three-hole | 10A three-hole | 10A three-hole | 10A three-hole |
Control cabinet dimension | Depth*Width*Height 520*350*618 mm | Depth*Width*Height 520*350*618 mm | Depth*Width*Height 520*350*618 mm | Depth*Width*Height 520*350*618 mm |
External dimensions of a single tank (w/o exhaust condenser) | Height*Diameter 357*100 mm | Height*Diameter 410*210 mm | Height*Diameter 520*250 mm | Height*Diameter 620*280 mm |
Control cabinet net weight | 35 kg | 35 kg | 35 kg | 35 kg |
Tank net weight | 2 kg | 8 kg | 11 kg | 15 kg |
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