Intelli Nuclease ELISA Kit

The Intelli nuclease ELISA kit is used to quantitatively detect the content of omnipotent nuclease residues in samples. It can be used for Duoning Intelli nuclease, and for omnipotent nuclease of other brands.

Specifications

Detection Method	Detection Type	Detection Scope
Colorimetry (one-step method)	Sandwich ELISA (quantitative)	0.2 ng/mL-20 ng/mL

Sensitivity	Estimated duration	Storage condition
0.06 ng/mL	2 hours and 15 minutes	2-8℃

Detection principle

The Intelli nuclease ELISA kit adopts double-antibody sandwich principle, and the anti-omnipotent nuclease antibody is coated on the ELISA plate, the sample to be tested is added and washed, and then the antibody labeled with horseradish peroxidase (HRP) is added for incubation to form an antibody-antigen-enzyme-labeled antibody complex, after thorough washing, the substrate TMB is added for color development, and the substrate TMB is transformed from colorless to blue under the catalysis of peroxidase, and is terminated by acid solution, finally forming a yellow clear liquid. There is a positive correlation between the color intensity and the content of omnipotent nuclease in the sample to be tested.

Standard curve

Intelli Nuclease ELISA Kit

Linear Range & Recovery

Theoretical conc. ng/mL	OD		Calibrated OD	Measured value ng/mL	Linear correlation coefficient (R²)	Recovery	Test result
20.00	2.767	2.754	2.725	19.53	1.0000	97.6%	Pass
1.00	0.324	0.325	0.289	0.97		96.8%
0.50	0.184	0.180	0.146	0.50		99.3%
0.33	0.132	0.128	0.094	0.33		100.0%
0.25	0.106	0.105	0.070	0.25		99.7%
0.20	0.088	0.085	0.051	0.18		91.5%

LoD

Detection times	OD	Calibrated OD	Mn	SD	Mn+2SD	LoD ng/mL	Test result
1	0.029	-0.007	-0.0067	0.0007	-0.0053	<0	Pass
2	0.029	-0.007
3	0.029	-0.007
4	0.029	-0.007
5	0.029	-0.007
6	0.031	-0.005
7	0.029	-0.007
8	0.029	-0.007

LLoD

Detection times	OD	Calibrated OD	Measured value ng/mL	Mn	SD	CV	Test result
1	0.090	0.054	0.19	0.1976	0.0039	2.0%	Pass
2	0.092	0.056	0.20
3	0.090	0.054	0.19
4	0.091	0.055	0.20
5	0.093	0.057	0.20
6	0.092	0.056	0.20
7	0.091	0.055	0.20
8	0.090	0.054	0.19

Compatibility test with omnipotent nuclease of other brands

Competitor	Brand H	Brand S	Brand Y 1	Brand Y2	Brand B
Compatibility	***	***	**	***	***

Note: Three stars is a perfect fit, and so on

Precision Test

Sample (ng/mL): 0.75
Reagent lot #	Test times	OD	Calibrated OD	Determination value ng/mL	Intra-run CV	Inter-run CV	Test result
Lot 1	1	0.248	0.212	0.71	3.2%	3.0%	Pass
	2	0.258	0.222	0.74
	3	0.249	0.213	0.71
	4	0.255	0.219	0.73
	5	0.245	0.209	0.70
	6	0.264	0.228	0.76
	7	0.246	0.210	0.70
	8	0.246	0.210	0.70
Lot 2	1	0.270	0.241	0.74	1.9%
	2	0.267	0.238	0.73
	3	0.268	0.239	0.73
	4	0.264	0.235	0.72
	5	0.268	0.239	0.73
	6	0.269	0.240	0.74
	7	0.273	0.244	0.75
	8	0.278	0.249	0.77
Lot3	1	0.305	0.278	0.73	2.0%
	2	0.316	0.289	0.76
	3	0.318	0.291	0.76
	4	0.316	0.289	0.76
	5	0.323	0.296	0.78
	6	0.315	0.288	0.76
	7	0.310	0.283	0.74
	8	0.311	0.284	0.75

Sample (ng/mL): 3
Reagen lot #	Test times	OD	Calibrated OD	Determination value ng/mL	Intra-run CV	Inter-run CV	Test result
Lot 1	1	0.797	0.761	2.75	4.2%	3.9%	Pass
	2	0.794	0.758	2.74
	3	0.872	0.836	3.08
	4	0.857	0.821	3.01
	5	0.839	0.803	2.93
	6	0.862	0.826	3.03
	7	0.830	0.794	2.89
	8	0.833	0.797	2.91
Lot 2	1	0.867	0.838	2.81	1.8%
	2	0.875	0.846	2.84
	3	0.862	0.833	2.79
	4	0.860	0.831	2.78
	5	0.889	0.860	2.90
	6	0.874	0.845	2.84
	7	0.877	0.848	2.85
	8	0.898	0.869	2.94
Lot 3	1	1.039	1.012	3.03	2.7%
	2	1.007	0.980	2.92
	3	1.070	1.043	3.15
	4	1.034	1.007	3.01
	5	1.022	0.995	2.97
	6	1.066	1.039	3.13
	7	1.038	1.011	3.03
	8	1.016	0.989	2.95

Sample (ng/mL): 12
Reagent lot #	Test times	OD	Calibrated OD	Determination value ng/mL	Intra-run CV	Inter-run CV	Test result
Lot 1	1	2.145	2.109	11.10	4.6%	7.2%	Pass
	2	2.256	2.220	12.21
	3	2.211	2.175	11.74
	4	2.217	2.181	11.80
	5	2.224	2.188	11.88
	6	2.294	2.258	12.62
	7	2.128	2.092	10.94
	8	2.214	2.178	11.77
Lot 2	1	2.284	2.255	11.02	2.0%
	2	2.321	2.292	11.35
	3	2.331	2.302	11.44
	4	2.331	2.302	11.44
	5	2.352	2.323	11.63
	6	2.314	2.285	11.28
	7	2.305	2.276	11.21
	8	2.277	2.248	10.96
Lot 3	1	2.528	2.501	11.67	5.9%
	2	2.639	2.612	12.78
	3	2.598	2.571	12.36
	4	2.742	2.715	13.90
	5	2.589	2.562	12.27
	6	2.715	2.688	13.60
	7	2.695	2.668	13.38
	8	2.674	2.647	13.15

Ordering information

Product Name	Specification	Part No.
Intelli Nuclease ELISA Kit	96 wells/kit	DNRG030301