CHO Stable Transfected Cell Culture Media
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DN Feed Series

DN feed 1+DN feed B

 

DN feed 1 is animal-free, protein-free, chemical-defined (CD) high-performance feed media, can be used for fed-batch culture of therapeutic protein product-producing Chinese hamster ovary (CHO) cells, through R&D to commercial manufacturing. DN feed 1 needs to be combined use with DN feed B, added to culture simultaneously.

 

Case study


 

DN补料系列

Bispecific antibody expression performance of CHOS cell with various cell culture media.



DN feed 2+DN feed B2

 

DN feed 2 is animal-free, protein-free, chemical-defined (CD) high-performance feed media, can be used for fed-batch culture of therapeutic protein product-producing Chinese hamster ovary (CHO) cells, through R&D to commercial manufacturing. DN feed 2 needs to be combined use with DN feed B2, added to culture simultaneously.

 

Case study


 

DN补料系列

Cell growth and product expression with DN feed 2 + DN feed B2 vs. other commercial feed media






How to use

We recommend to start adding feed media during middle-late stage of cell exponential growth stages, usually on day 3 when cell density reaches 2.0~3.0×106 cells/mL, followed by add every other day with 3%±0.3%. Users can optimize the feed strategy based on the cell lines.

 

DNFeed Series can be used for cell lines using GS and dhfr screening systems, including CHO-K1, CHO-S, DG44, CHOZN and CHOK1SV.


Ordering information

Product Name

Product No.

Spec.

Form

Shelf Life

DN feed 1

CDF1-001

1L

Liquid

6 months

CDFP1-001

100g

Powder

2 years

CDFP1-010

1kg

Powder

2 years

CDFP1-050

5kg

Powder

2 years

DN feed B

CDB1-001

300mL

Liquid

6 months

CDBP1-001

100g

Powder

2 years

CDBP1-010

1kg

Powder

2 years

DN feed 2

FT002-1L

1L

Liquid

6 months

FP002-100g

100g

Powder

2 years

FP002-1kg

1kg

Powder

2 years

FP002-5kg

5kg

Powder

2 years

DN feed B2

FT003-300mL

300mL

Liquid

6 months

FP003-100g

100g

Powder

2 years

FP003-1kg

1kg

Powder

2 years


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