CHO Transient Transfection Cell Culture Medium

Duoning CHO transfection medium includes the Transpro series and DN feed series media products. Based on the production process characteristics of transient transfection produced proteins, Duoning medium product development team focuses on the research of culture medium formula mechanism, CHO cell metabolism mechanism, and transient transfection mechanism, and through rational experimental design, we have successfully designed and developed the Transpro series & DN feed series of culture medium products. Duoning CHO transfection medium is suitable to produce antibodies and recombinant proteins by transient transfection of CHO cell lines, such as ExpiCHOS, CHOS and CHOK1. Together with the upstream and downstream bio-pharmaceutical process products from Duoning, we can provide customers with complete solutions for diﬀerent bioprocessing scenarios.

CHO Cell Transient Transfection Culture Medium

Product Features

Completely chemically deﬁned (CD) medium

Support high cell density suspension culture, transient transfection, and achieve high productivity Applicable to diﬀerent CHO cell types (ExpiCHOS, CHOS, CHOK1, etc.)

Provide convenient preparation methods for GMP production

Products have independent intellectual property rights

Product Benefits

Compatible with high cell density growth and transient transfection of HEK293 cells and CHO cells

Simple process, no need for medium exchange , the transfection operation can be started after direct dilution. And the feeding process after transfection is simple, only two feeding operations are needed to meet the nutri-tional requirements of cell culture for more than 11 days.

Combined with the DN feed series, the antibody titer can be signiﬁcantly improved and productivity above g/L is achievable.

Supports the adaptation of adherent cells into suspension cells. Generally, it can be adapted after 3 passages from other suspension medium.

Provide full technical support services, and the application team has supported and served more than 100 customers.

Product Performance Data

Case study 1: Protein production

Medium combination: Transpro CD 01 plus/Transpro CD 02+DN feed 2+DN feed B2+transient transfection supplement-02

Recommended cells: ExpiCHO, CHOS, CHOK1 and others

Ordering Information

Product type	Product name	Specification	Product number	Product form
Basal medium	Transpro CD 01 medium	100 g	MP004-100g-02	Dry powder
		1 kg	MP004-1kg-02
		5 kg	MP004-5kg-02
		10 kg	MP004-10kg-02
		25 kg	MP004-25kg-02
	Transpro CD 01 plus medium	100 g	MP027-100g-02
		1 kg	MP027-1kg-02
		5 kg	MP027-5kg-02
		10 kg	MP027-10kg-02
		25 kg	MP027-25kg-02
	Transpro CD 02 medium	100 g	PB007-100g-02
		1 kg	PB007-1kg-02
		5 kg	PB007-5kg-02
		10 kg	PB007-10kg-02
		25 kg	PB007-100g-02
Feed medium	DN feed 2 medium	1 L	M021-1L-02
		1 kg	FP002-1kg-02
		5 kg	FP002-5kg-02
		10 kg	FP002-10kg-02
		25 kg	FP002-25kg-02
	DN feed B2 medium	100 g	FP003-100g-02
		1 kg	FP003-1kg-02
		5 kg	FP003-5kg-02
		10 kg	FP003-10kg-02
		25 kg	FP003-25kg-02
Basal medium	Transpro CD 01	500 ml	MS002-500ml-02	Liquid
	Transpro CD 01	1 L	MS002-1L-02
	Transpro CD 01 plus	500 ml	M021-500ml-02
	Transpro CD 01 plus	1 L	M021-1L-02
	Transpro CD 02	500 ml	LB014-500ml-02
	Transpro CD 02	1 L	LB014-1L-02
Feed medium	DN feed 2	100 ml	FT002-100ml-02
		500 ml	FT002-500ml-02
		1 L	FT002-1L-02
	DN feed B2	100 ml	FT003-100ml-02
		500 ml	FT003-500ml-02
		1 L	FT003-1L-02