His-tag protein purification resin

His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

His-tag protein purification resin (GP-IDA) uses super-macro-porous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni²⁺. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.

His-tag protein purification resin (CP-NTA) uses macro-porous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).

His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni²⁺ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni²⁺. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.

Product	His tag protein purification resin (HP/CP/GP-IDA）			His tag protein purification resin (HP/CP/GP-NTA）			His tag protein purification resin (HP/CP/GP-TED）
Matrix	Polyacrylate hard gel			Polyacrylate hard gel			Polyacrylate hard gel
Ligand	IDA			NTA			TED
Average particle size	70 μm			70 μm			70 μm
Dynamic capacity mg/mL	HP-IDA	CP-IDA	GP-IDA	HP-NTA	CP-NTA	GP-NTA	HP-TED	CP-TED	GP-TED
	≥35	≥25	≥20	≥20	≥15	≥10	≥20	≥15	≥10
Max flowrate	1500cm/h			1500cm/h			1500cm/h
Max Pressure	1MPa			1MPa			1MPa
Storage	4-30°C (20% ethanol)			4-30°C (20% ethanol)			4-30°C (20% ethanol)
Reducing agent resistance	No			β-Me≤100mM; DTT≤5mM			β-Me≤100mM; DTT≤5mM; TCEP≤5mM
Feature	High protein capacity			Low metal ion leaching rate			Resistant to 10 mM ethylenediaminetetraacetic acid (EDTA), 0.5M NaOH, and 6M guanidine-HCl for 24 hours; resistant to 500mM imidazole, 100mM ethylenediaminetetraacetic acid (EDTA) for 2 hours

Bogen can provide different ligand metal chelating resin with different pore sizes according to customer needs.

His tag protein purification resin

For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu²⁺, Zn²⁺, Co²⁺ and other transition metal ions.

Product Features

Product	His tag protein purification resin (HP/CP/GP-IMAC）	His tag protein purification resin (IMAC）
Matrix	Polyacrylate hard gel	Agarose soft gel
Ligand	NTA	NTA
Average particle size	70μm	90μm
Max flowrate	1500cm/h	500cm/h
Max Pressure	1MPa	0.3MPa
Storage	4-8℃ (20% ethanol）	4-8℃ (20% ethanol)
Feature	Low metal ions leaching rate, and the type of metal ions can be freely selected

Case Study

Purifying His-fusion protein from supernatant of E.Coli lysate.

His-tag protein purification resin

Ordering Information

HP series

Product Name	Part No.	Specification	Product Name	Part No.	Specification
His tag protein purification resin (HP-IDA)	BG11-1110-02	50mL	His tag protein purification resin (HP-NTA)	BG11-1111-02	50mL
	BG11-1110-04	500mL		BG11-1111-04	500mL
	BG11-1110-05	1L		BG11-1111-05	1L
	BG11-1110-07	10L		BG11-1111-07	10L
	BG11-1110-08	20L		BG11-1111-08	20L
His tag protein purification resin (HP-TED)	BG11-1113-02	50mL	His tag protein purification resin (HP-IMAC)	BG11-1101-02	50mL
	BG11-1113-04	500mL		BG11-1101-04	500mL
	BG11-1113-05	1L		BG11-1101-05	1L
	BG11-1113-07	10L		BG11-1101-07	10L
	BG11-1113-08	20L		BG11-1101-08	20L

CP series

Product Name	Part No.	Specification	Product Name	Part No.	Specification
His tag protein purification resin (CP-IDA)	BG11-2110-02	50mL	His tag protein purification resin (CP-NTA)	BG11-2111-02	50mL
	BG11-2110-04	500mL		BG11-2111-04	500mL
	BG11-2110-05	1L		BG11-2111-05	1L
	BG11-2110-07	10L		BG11-2111-07	10L
	BG11-2110-08	20L		BG11-2111-08	20L
His tag protein purification resin (CP-TED)	BG11-2113-02	50mL	His tag protein purification resin (CP-IMAC)	BG11-2101-02	50mL
	BG11-2113-04	500mL		BG11-2101-04	500mL
	BG11-2113-05	1L		BG11-2101-05	1L
	BG11-2113-07	10L		BG11-2101-07	10L
	BG11-2113-08	20L		BG11-2101-08	20L

Agarose series

Product Name	Part No.	Specification	Product Name	Part No.	Specification
His tag protein purification resin (IDA)	8G11-0010-02	50mL	His tag protein purification resin (NTA)	BG11-0011-02	50mL
	BG11-0010-04	500mL		BG11-0011-04	500mL
	BG11-0010-05	1L		BG11-0011-05	1L
	BG11-0010-07	10L		BG11-0011-07	10L
	BG11-0010-08	20L		BG11-0011-08	20L
His tag protein purification resin (TED)	BG11-0013-02	50mL	His tag protein purification resin (IMAC)	BG11-0001-02	50mL
	BG11-0013-04	500mL		BG11-0001-04	500mL
	BG11-0013-05	1L		BG11-0001-05	1L
	BG11-0013-07	10L		BG11-0001-07	10L
	BG11-0013-08	20L		BG11-0001-08	20L

His tag protein purification prepacked column

Product Name	Specification	Applicable Molecular Weight	Capacity	Max Pressure	Max Flow rate	Application/Feature
His tag protein purification column (HP-IDA)	5mL 10mL	≤200KD	≥35mg/mL	1MPa	1000cm/h	For His tag protein purification, high dynamic capacity, high maximum pressure
His tag protein purification column (HP-NTA)	5mL 10mL	≤200KD	≥20mg/mL	1MPa	1000cm/h	For His tag protein purification, low ligand leaching rate, high maximum pressure
His tag protein purification column (HP-TED)	5mL 10mL	≤200KD	≥20mg/mL	1MPa	1000cm/h	For purification of His-tagged proteins expressed in eukaryotic cells, resistant to a certain concentration of EDTA, directly cleaned with NaOH, high maximum pressure
His tag protein purification column (GP-IDA)	5mL 10mL	≥150KD	≥20mg/mL	1MPa	1000cm/h	For purification of His-tagged proteins with extremely large molecular weight, or to capture His-tagged proteins from relatively viscous liquids.
His tag protein purification column (GP-NTA)	5mL 10mL	≥150KD	≥10mg/mL	1MPa	1000cm/h	For purification of His-tagged proteins with extremely large molecular weight, or to capture His-tagged proteins from relatively viscous liquids, low ligand leaching rate, high maximum pressure
His tag protein purification column (GP-TED)	5mL 10mL	≥150KD	≥10mg/mL	1MPa	1000cm/h	Purification of ultra-large molecular weight His-tagged proteins expressed in eukaryotic cells, withstands a certain concentration of EDTA, can be directly cleaned with NaOH, and has high maximum pressure
His tag protein purification column (IDA)	5mL 10mL	≤200KD	≥40mg/mL	0.3MPa	600cm/h	For purification of His tag protein, high dynamic capacity
His tag protein purification column (NTA)	5mL 10mL	≤200KD	≥20mg/mL	0.3MPa	600cm/h	For purification of His tag protein, low ligand leaching
His tag protein purification column (TED)	5mL 10mL	≤200KD	≥15mg/mL	0.3MPa	600cm/h	For purification of His-tagged proteins expressed in eukaryotic cells. It can withstand a certain concentration of EDTA and can be directly cleaned with NaOH.