Stainless Steel Microbial Fermentation System

With years of manufacturing experience, Duoning provides customers with 15 L, 30 L, 50 L, 100 L modular-designed pilot-scale system for microbial fermentation, and customized >200 L production-scale fermentation system. The pilot fermentation systems are widely used in the culture of bacteria, cocci, filamentous bacteria, plant cells, yeast, for optimizing the conditions for large-scale production process, or for clinical material manufacturing. The expert team can provide customers with process design, pipeline construction, system integration and manufacturing, control software development and other services.

System Features

Modular design, space saving, small footprint;

Sterilization-in-place, easy to use, labor saving;

Equipped with in-place sampling valve for aseptic sampling;

The rational use of axial flow impeller and radial flow impeller provides a high oxygen transfer rate to ensure the demand for high-density culture;

Electric heating for temperature control, achieving rapid heating and cooling, suitable for the production of temperature-induced products;

Enables remote monitoring and communication;

Temperature control using jacket and tank internal dual PID control loop, achieving stable temperature control, high accuracy within ±0.1℃;

Pin type feeding port, easy to use, optional for sterilization-in-place feeding valve group;

Standard configuration with rotameter, optional air/oxygen mixer and mass flowmeter;

Air intake/exhaust filter sterilization can be carried out independently to meet the requirements of filter replacement during the culture process;

Pipeline and frame adopt open design, easy to use and maintain;

Pipeline adopts 3A standard, comply with GMP and BPE requirements;

All valves or instruments can be switched between automatic and manual mode with one click control on the main screen;

Instrument/valve control mode: manual control mode can be selected in the main control screen - you can manually switch the valve or instrument; Or automatic control mode: PID control can be selected

Dissolved oxygen control: DO can be controlled by selecting AIR/O₂ flow rate or stirring speed;

Multi-user authorization: multi-level user authorization with password protection to avoid process parameters and data been arbitrarily modified;

Software allows for parameter setting, display control, parameters monitoring, curve analysis, data storage, and output printing;

Multiple levels of control and viewing authorizations.

Technical parameters

Tank body	Total volume	15 L	30 L	50 L	100 L
	Working volume	10 L	8-22 L	15-35 L	25 – 72 L
	Diameter-height ratio	1:2.2	1:2.2	1:2.2	1:2.2
	Material	316L SS
	Head plate	316L SS, 4 pieces
	Manufacturing standard	ASME, PED, GB D1/D2, ISO9001:2000
		Material 316L stainless steel; Jacket 304 stainless steel Polishing accuracy of the surface in contact with the material Ra<0.4 Design pressure: 0.3 MPa, working pressure: 0.25 MPa Connection configuration: safety glass window, side view window, feed valve group port, foam sensor port, pressure sensor port, in-place sampling port, air intake port, bottom valve, inoculation valve group port, base feed port, safety bursting disc
Stir	Type	Top stir, mechanical seal, PID control
	Display	Digital display, ±1 rpm
	Stir speed	25~800 rpm, ±1 rpm
	Impeller	Six-blade impeller
Detection probe	pH	2~12, PID control, accuracy: ±0.01, correlate with base and acid feed pump
	DO	0～200%, PID control, accuracy: ±1%, correlate with gas supply flow rate and stir speed (optional）
	Liquid level	SS probe, PID control
	Temperature	Pt100, PID Control, Digital display, ±0.1℃
Gas control	Flowrate measurement	Thermal mass flow controller (optional)
	Gas	Four-gas control system (N_2,O_2,CO_2,Air) , solenoid valve control
	Intake filter	0.2 μm, PTFE
	Exhaust filter	0.2 μm, PTFE
	Exhaust condenser	316L SS
	Pressure regulation	Automatic pressure regulating system, including pressure sensor, and regulating valve
Liquid feed	Peristaltic pump	Variable speed pump, and flow is accumulated,
Temperature	Control method	PID, Automatic sterilization; The temperature is controlled by the external heater and the cooling water under the control of the control system
	Control accuracy	Display accuracy: 0.1℃, control accuracy: ±0.1℃
	Control range	5 ℃~65 ℃ above cooling water temperature
Control system		Siemens programmable logic controllers for industrial use
Optional configurations		Weighing, tank lid automatic lifting
Water, electricity, gas requirements		Cooling water pressure: 0.15MPa
		Cooling water temperature: not higher than 30 ℃
		Input air pressure: 0.2MPa (subject to drying and pre-filtration)
		Input steam pressure: 0.18MPa (must be pre-filtered)

For more information about custom systems, please contact your local representatives or technical support team of Duoning.