Transpro Series HEK293 Cell Culture Medium

Duoning HEK293 culture medium includes Transpro series and Transpro feed series. Based on the production process characteristics of transient transfection produced proteins and virus packaging, Duoning medium product development team focused on the research of culture medium formula mechanism, HEK293 cell metabolism mechanism, and transient transfection mechanism, and through rational experimental design, we have successfully designed Transpro series & Transpro feed series products. Duoning HEK293 culture medium is suitable for using diﬀerent types of HEK293 cells (Expi293F, HEK293 and 293F, etc.) for protein expression and virus packaging.

HEK293 Cell Culture Medium

Product Features

Completely chemically deﬁned (CD) medium

Support high cell density suspension culture, transient transfection, and achieve high productivity Applicable to diﬀerent HEK293 cell types (Expi293F, HEK293, 293F, etc.)

Suitable for use as growth medium for HEK293 cells and production medium for antibody or recombinant protein, adeno-associated virus (AAV), and lentivirus (LV) virus packaging.

Provide convenient preparation methods for GMP production

Products have independent intellectual property rights

Product Benefits

Simple process, no need for medium exchange by centrifugation, the transfection operation can start after direct dilution.

Combined with the matching feed medium series, it can signiﬁcantly improve expression eﬃciency. Supports the adaption of adherent cells into suspension cells. Generally, it can be adapted after 3 passages from other suspension medium.

Can be used as a diluent in the transfection reagent preparation process, streamlining transfection and boosting eﬃciency.

Provide full technical services and can help to optimize transient transfection parameters and culture process according to customer needs.

Product Performance Data

Case study 1:

reProtein production by transient transfection Medium combination: Transpro CD 01 plus/Transpro CD 02+Transpro feed 1

Recommended cells: Expi293, 293F and others

Case study 2:

Associated-Adeno Virus (AAV) production Medium combination: Transpro CD 01/Transpro CD 01 plus + Transpro feed 1

Recommended cells: HEK293, 293T and others

Transpro Series HEK293 Cell Culture Medium

Case study 3:

Lentivirus (LV) production Medium combinations: Transpro CD 01/Transpro CD 01 plus + Transpro feed 1

Recommended cells: HEK293, 293T and others

Ordering Information

Product type	Product name	Specification	Product number	Product form
Basal medium	Transpro CD 01 medium	100 g	MP004-100g-02	Dry powder
		1 kg	MP004-1kg-02
		5 kg	MP004-5kg-02
		10 kg	MP004-10kg-02
		25 kg	MP004-25kg-02
	Transpro CD 01 plus medium	100 g	MP027-100g-02
		1 kg	MP027-1kg-02
		5 kg	MP027-5kg-02
		10 kg	MP027-10kg-02
		25 kg	MP027-25kg-02
	Transpro CD 02 medium	100 g	PB007-100g-02
		1 kg	PB007-1kg-02
		5 kg	PB007-5kg-02
		10 kg	PB007-10kg-02
		25 kg	PB007-25kg-02
	Transpro CD 01 plus	500 ml	M021-500ml-02	Liquid
	Transpro CD 01 plus	1 L	M021-1L-02
	Transpro CD 01	500 ml	MS002-500ml-02
	Transpro CD 01	1 L	MS002-1L-02
	Transpro CD 02	500 ml	LB014-500ml-02
	Transpro CD 02	1 L	LB014-1L-02
Feed medium	Transpro feed 1 medium	100 g	PF003-100g-02	Dry powder
		1 kg	PF003-1kg-02
		5 kg	PF003-5kg-02
		10 kg	PF003-10kg-02
		25 kg	PF003-25kg-02
	Transpro feed 1	100 ml	F002-100ml-02	Liquid
		500 ml	F002-500ml-02
		1 L	F002-1L-02