Max Series Cell Culture Media

Maxgrow 201 cell culture media

Maxgrow 201 media can be used for cell banking and expansion culture through process development to commercial manufacturing, for Chinese ovary cells (CHO) – based therapeutic protein production.

Maxpro 303 cell culture media

Maxpro 303 cell culture media can be used for batch, fed-batch and perfusion cell culture through process development to commercial manufacturing, for Chinese ovary cells (CHO) – based therapeutic protein production.

Maxfeed 403A1+ Maxfeed 403B1 feed media

Maxfeed 403A1+ Maxfeed 403B1 feed media can be used for fed-batch cell culture through process development to commercial manufacturing, for Chinese ovary cells (CHO) – based therapeutic protein production.

Maxgrow Supplement

Maxgrow Supplement if animal-free, protein-free, chemical-defined cell culture media supplement. Adding Maxgrow Supplement with appropriate ratio during monoclonal screening can reduce the use of serum/serum substitutes, and improve clone formation; Adding Maxgrow Supplement with appropriate ratio during CHO cell culture process development and manufacturing can significantly improve the cell density and viability of difficult-to-grow CHO cells/clones, thus improving the process performance.

How to use

We recommend to use 201 as seed-train media after cell thawing, and use seeding density of 0.3~0.8×10⁶ cells/mL for cell passage and expansion. For N-1 culture, change to 303 as basal media, and after expanding to desire seed volume, with seeding density been controlled as 1.0~2.0×10⁶ cells/mL, perform fed-batch culture using 403A1+ 403B1 as feed. it is recommended to feed every other day starting from Day2 at 3%+0.3%, and users can also optimize the feed strategy based on the cell line used. Add 0.5% Maxgrow Supplement (5mL/L) to 303 media can further improve culture performance.

Max series can be used for cell lines using GS screening systems, including CHO-K1, CHO-S, and CHOK1SV.

Benefits:

Ÿ Animal-free

Ÿ Protein-free

Ÿ Chemical-defined

Cell growth and antibody expression of CHOK1 cell